BBa_K1362108
BBa_K1362108 Version 1 (Component)
RBS + NpuDnaE N&C-intein coexpression RFC[105] assembly construct (with His6)

BBa_K1362161
BBa_K1362161 Version 1 (Component)
RBS + Gp41-1 C-intein RFC[105] assembly construct (with His6)

BBa_K1362166
BBa_K1362166 Version 1 (Component)
RBS + Gp41-1 N-intein RFC[105] assembly construct with additional C-teminal insertion site

BBa_K1362167
BBa_K1362167 Version 1 (Component)
RBS + Gp41-1 C-intein RFC[105] assembly construct with additional N-teminal insertion site

BBa_G0007
BBa_G0007 Version 1 (Component)
Short sequence to promoter addition of 3' A overhang during PCR

BBa_G0008
BBa_G0008 Version 1 (Component)
Short sequence to promoter addition of 3' A overhang during PCR

NheI
BBa_B0045 Version 1 (Component)
NheI restriction enzyme site (XbaI, SpeI compatible overhang)

SspDnaX(N)
BBa_K1362110 Version 1 (Component)
RBS + SspDnaX N-intein RFC[105] assembly construct (with His6)

BBa_K1362111
BBa_K1362111 Version 1 (Component)
RBS + SspDnaX C-intein RFC[105] assembly construct (with His6)

SspGyrB(N)
BBa_K1362120 Version 1 (Component)
RBS + SspGyrB N-intein RFC[105] assembly construct (with His6)

BBa_K1362160
BBa_K1362160 Version 1 (Component)
RBS + Gp41-1 N-intein RFC[105] assembly construct (with His6)

AvrII
BBa_B0048 Version 1 (Component)
AvrII restriction enzyme site (XbaI, SpeI compatible overhang)

NsiI
BBa_B0046 Version 1 (Component)
NsiI restriction enzyme site (PstI compatible overhang)

MfeI
BBa_B0047 Version 1 (Component)
MfeI restriction enzyme site (EcoRI compatible overhang)

BBa_K1362300
BBa_K1362300 Version 1 (Component)
NpuDnaE N-Intein RFC[105] assembly construct (with His6)

1Gate-NpuD
BBa_K1362100 Version 1 (Component)
RBS + NpuDnaE N-intein RFC[105] assembly construct (with His6)

<- BsaI
BBa_K1362423 Version 1 (Component)
BsaI reverse restriction site for RFC[105] cloning

BsaI ->
BBa_K1362424 Version 1 (Component)
BsaI restriction site for RFC[105] cloning

RFC105 NC
BBa_K1362417 Version 1 (Component)
C-terminal GG-linker overhang GG<u>TGGT</u>=Gly+Gly RFC[105] overhang NC

RFC105 Z
BBa_K1362421 Version 1 (Component)
C-terminal stop overhang TAAT=STOP+1/3Stop RFC[105] overhang Z

RFC105 A
BBa_K1362414 Version 1 (Component)
N-terminal start overhang (T)(A)-(G)ATG=RBS+Start RFC[105] A

RFC105 NN
BBa_K1362415 Version 1 (Component)
N-terminal splicing overhang TGCT=Cys+1/3 Leu|Ile RFC[105] overhang NN

RFC105 CC
BBa_K1362419 Version 1 (Component)
C-terminal splicing overhang CAAC=1/3?+Asn RFC[105] standard overhang CC

RFC[105] E
BBa_K1362420 Version 1 (Component)
C-terminal non-splicing overhang AGGC=1/3Ala+Gly RFC[105] E

RFC105 CN
BBa_K1362418 Version 1 (Component)
N-terminal GG-linker overhang <u>GGTG</u>=Gly+1/3Gly RFC[105] overhang CN

RFC105 NN*
BBa_K1362416 Version 1 (Component)
N-terminal non-splicing overhang GGCT=Gly+1/3Leu|Ile RFC[105] overhang NN*

CircNpu
BBa_K1362000 Version 1 (Component)
NpuDnaE intein RFC[105] circularization construct

CircNpu*
BBa_K1362001 Version 1 (Component)
NpuDnaE intein RFC[105] circularization construct (with FLAG and Smt3)

BBa_K1362052
BBa_K1362052 Version 1 (Component)
Carrier for RFC[105] C-terminal inserts

BBa_K1362054
BBa_K1362054 Version 1 (Component)
SsrA degradation tag as RFC[105] C-terminal insert

N-carrier
BBa_K1362053 Version 1 (Component)
Carrier for RFC[105] N-terminal inserts

RFC105 A
BBa_K1362440 Version 1 (Component)
N-terminal start overhang (T)(A)-GATG=RBS+Start RFC[105] A

BBa_K1362055
BBa_K1362055 Version 1 (Component)
MinD MTS as RFC[105] C-terminal insert

BBa_K1362106
BBa_K1362106 Version 1 (Component)
RBS + NpuDnaE N-intein RFC[105] assembly construct with additional C-teminal insertion site

1GateNpuE
BBa_K1362101 Version 1 (Component)
RBS+NpuDnaE C-intein RFC[105] assembly construct (with His6)

BBa_K1362107
BBa_K1362107 Version 1 (Component)
RBS + NpuDnaE C-intein RFC[105] assembly construct with additional N-teminal insertion site

1NpuE(N*)
BBa_K1362102 Version 1 (Component)
RBS + NpuDnaE N-intein RFC[105] non-splicing control construct (with His6)

1NpuE(C*)
BBa_K1362103 Version 1 (Component)
RBS + NpuDnaE C-intein RFC[105] non-slicing control construct (with His6)

BBa_K1362056
BBa_K1362056 Version 1 (Component)
Myristoilation signal sequence as RFC[105] N-terminal insert

BBa_K1362057
BBa_K1362057 Version 1 (Component)
NLS as RFC[105] N-terminal insert

BBa_K1362058
BBa_K1362058 Version 1 (Component)
PelB leading sequence as RFC[105] N-terminal insert

BBa_K1362059
BBa_K1362059 Version 1 (Component)
PKI-NES as RFC[105] N-terminal insert

BBa_K1362060
BBa_K1362060 Version 1 (Component)
OmpA surface protein as RFC[105] N-terminal insert

BBa_K1362451
BBa_K1362451 Version 1 (Component)
N-terminal start overhang short

SspGyrB(C)
BBa_K1362121 Version 1 (Component)
RBS + SspGyrB C-intein RFC[105] assembly construct (with His6)

TerDnaE3(N
BBa_K1362130 Version 1 (Component)
RBS + TerDnaE3 N-intein RFC[105] assembly construct (with His6)

TerDnaE3(C
BBa_K1362131 Version 1 (Component)
RBS + TerDnaE3 C-intein RFC[105] assembly construct (with His6)

SspDnaB(N)
BBa_K1362140 Version 1 (Component)
RBS + SspDnaB N-intein RFC[105] assembly construct (with His6)

SspDnaB(C)
BBa_K1362141 Version 1 (Component)
RBS + SspDnaB C-intein RFC[105] assembly construct (with His6)

SspDnaB(N*
BBa_K1362142 Version 1 (Component)
RBS + SspDnaB N-intein RFC[105] non-splicing control construct (with His6)